Recombinant Human Cbl-c/CBL-3 Protein (His & GST Tag)

Recombinant Human Cbl-c/CBL-3 Protein (His & GST Tag)

SKU #	PKSH030531
Expression Host	Baculovirus-Insect Cells

Description

Synonyms	CBL-3,CBL-SL,RNF57
Species	Human
Expression Host	Baculovirus-Insect Cells
Sequence	Met 1-Ala474
Accession	AAH28915.1
Calculated Molecular Weight	80.2 kDa
Observed Molecular Weight	66 kDa
Tag	N-His-GST
Bio-activity	Not validated for activity

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin	< 1.0 EU per g of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 8.0 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

CBL proteins, such as Cbl-c, are phosphorylated upon activation of a variety of receptors that signal via protein tyrosine kinases. Through interactions with proteins containing SRC homology-2 (SH2) and SH3 domains, CBL proteins modulate downstream cell signaling. Cbl-c is a member of the Cbl family of E3 ubiquitin ligases. Expression of Cbl-c gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for Cbl-c gene.